| 1. | 1 % of amino acid ( aa ) sequence homologies compared with those of toledo , respectively
3 。 4 、氨基酸变异位点多集中于序列中部，两端变异较少。 |
| 2. | Comparing the sequences of ses , we found that the members of ses share the high sequence homology , and some active sites are conservative
将sed与seb 、 sec3的氨基酸序列进行比较分析，发现某些与tcr结合的活性位点比较保守。 |
| 3. | Chicken tyrp1 exons show 70 . 1 % nucleotide sequence homology with human exons . fish result shows that tyrp1 locates on the long arm of z and w near to the centromere
用荧光原位杂交进行tyrp1的染色体定位，首次将tyrp1定位在z和w染色体长臂近着丝粒的位置。 |
| 4. | After sequencing of 84 cdna clones and removing redundant cdnas , we obtained 36 cold - regulated unique cdna clones . 12 cdna clones were expected to be novel genes , because no sequence homology with any known sequences was found in genbank databases
全长基因ej175共有603bp ，对其可读阅读框架进行分析，从57 - 515位核苷酸的一段序列，包含了起始密码子和终止密码子，编码152个氨基酸的多肽。 |
| 5. | The cloned segment a contains 3259 nucleotides in full - length and includes two partially overlapping open reading fragments ( orf1 and orf2 ) flanked by 5 " and 3 " noncoding regions ( ncr ) . the two strains shared high sequence homology with each other either at nucleotide or deduced amino acid level
序列测定结果表明，两株病毒的基因组a节段全长共3259个核苷酸，包括5 ’ 、 3 ’端的非编码区( ncr )和两个部分重叠的开放阅读框( orf1和orf2 ) 。 |
| 6. | Rap - specific gaps have no sequence homology with rasgap and other gaps such as rangap and rhogap . rap is the only one which does not have the invariant gin ( gin 61 in ras ) in switch ii which introduce the arginine finger on gap into active site of ras to complement catalytic machinery
由于rap1gap和rasgap及其它小分子g蛋白的gap (如rangap 、 rhogap )相比在序列上没有同源性，并且rap是唯一一个在switch上没有保守谷氨酰胺的小分子g蛋白。 |
| 7. | Strain 10010 belong to nocardiopsis , the sequence homology with n . lucentensis was 98 . 80 % . the two strains were similar to n . lucentensis . but they were different in colony colour , sugar of whole cell , menaquinones characteristics and dna g + c mol % . so they are maybe different species
菌株10001与菌株1001016srdna序列相似性为99 . 58 ，但菌株10010和菌株10002菌落颜色不同，且在细胞壁糖型、醌类及dnag + cmol等方面有差异，可能为不同种。 |
| 8. | The cloning cdna fragment was extracted from positive clones and sequenced . the results showed that the cdna fragment was 816bp in size , encoding a protein which included 272 amino acids . the sequence homology analysis was carried out via the software blast 2 . 0 network service in the four large databases - genbank , embl , ddbj , pdb , which had recorded 1 337 978 nucleotide and protein sequences . the results of the analysis indicated that the nucleotide homologous rates between the rubber tree etr and 15 recorded etrl of other plants ( mango , passion fruit , persia plum , strawberry , grape . . . etc ) were 75 % - 80 % ; the protein homologous rates between the rubber tree etrl and these recorded etrl genes were 90 % - 95 % . from the results mentioned above , we could confirm that the cdna of rubber tree etrl had been cloned
从阳性克隆子中提取克隆片段，经序列测定分析，结果表明，克隆片段的cdna大小为816bp ，编码的蛋白质包含272个氨基酸。基因序列通过blast2 . 0networkservice软件对genbank ， embl ， ddbj ， pdb四个大型数据库中记录的1337978条核酸和蛋白质序列进行序列相似性检索，结果表明与芒果、一西番莲、波斯梅、草毒、葡萄、西洋梨等15种已报道的植物的etrl基因cdnag的同源率为75 88 ；蛋白质氨基酸序列的同源率为90 95 ，表明本研究确实克隆到了橡胶树etri基因的cdna序列。 4 |
| 9. | Four colonies of transformed e . coli dh5 a with clear hydroiyzing zone on the chitin agar were obtained . the gene fragment in these isolates was identified by the methods of plasmid processing . dna sequencing analysis showed that sequence homology between pcr fragment and chromosomal dna of b . subtilis from 2599451 to 2812870 was 85 % , and was 30 % between the fragment and the genes encoding for chitinase of bacillus ( including b . subtilis ) in genebank
测序并序列比较结果表明该基因片段同已发表的枯草芽孢杆菌几丁质酶和内切葡聚糖酶编码幕因的克隆及重组芽抱杆菌的构建glyb一apre之间的同源性是最高的，为35 % ;同bacz ’了了ussp . bp23ce1b 、 b . p朋刀us内切葡聚糖酶和b . pol理vxap一1 ， 4一内切葡聚糖酶的编码基因的同源性只有27 % 。 |
| 10. | The nucleotide ( nt ) sequence of the insert in phz1754 is 2299bps in size . computer assisted analysis of the sequence revealed an open reading frame ( orf ) with a g + c content of 70 . 3 % that would encode a protein of 552 amino acids ( aa ) . the nt seque nce comparision revealed that the orf in the sequenced region exhibits 85 % dna sequence homology with the cholesterol oxidase gene choa of streptomyces sp
对phz1754进行外切核酸酶( exonuclease ， exo )顺序缺失，获得单向长度渐减重叠的系列突变体，核苷酸序列测定显示出该ecor - sal片段的精确大小为2299bps ， frameplot程序分析揭示出该区域一个完整的开放阅读框( orf )的存在，其大小为1656bps ， g + c含量为70 . 3 ，编码552个氨基酸，利用blastsearch程序将orf的核苷酸序列及推导的氨基酸序列与因特网上基因及蛋白质数据库进行综合比较，发现无论在核苷酸水平还是在蛋白水平上，该orf均与胆固醇氧化酶表现出同源性，而且与链霉菌胆固醇氧化酶同源性最高，说明该orf编码胆固醇氧化酶基因。 |
